WPC Dye for Sysmex 5-Part Hematology Analyzers e fana ka matheba a phahameng-a khethehileng a fluorescent bakeng sa mecha ea ho lemoha lisele tse tšoeu tsa lisele ho litsamaiso tse phahameng tsa tlhahlobo ea mali. E etselitsoe lerata le tlase la mokokotlo le ho kenella ka potlako ha lisele tsa lisele, reagent ena e etsa hore ho be le scattergram gating ea li-leucocyte tse sa sebetseng le lihlopha tsa lisele tsa atypical nakong ea ts'ebetso ea laser flow cytometry.
Li-reagents tsa Hematology Stain / Dye ke Eng?
Li-automatology hematology fluorescent dye reagents ke metsoako ea lik'hemik'hale tse metsi tse etselitsoeng{{0}li-speed automated cell counters. Ho fapana le matheba a "microscopic blood smear", litharollo tsena tsa mokelikeli li kena ka likamoreng tsa karabelo ea microfluidic, li kenella ka har'a lisele tsa sele, 'me li shebile li-nucleic acid tsa intracellular (DNA le RNA). Sephetho se hlahisoang ke khanya se lumella lisebelisoa tsa optical sensor ho arola lisele tsa lisele ho latela mahloriso a nucleic acid le ho rarahana ha ka hare.
Seabo ho Phapang ea Lisele / Ho Fumana
Nakong ea potoloho ea tlhahlobo, dae e khetha ka ho khetheha likhoele tsa intracellular nucleic acid. Ha o feta ka har'a semiconductor laser beam, likarolo tse tšoailoeng li ntša matšoao a fluorescent. Ka ho ts'oara matla a fluorescence ka lehlakoreng le ka pele le ka lehlakoreng la ho hasanya leseli, sistimi e rarolla lihlopha tsa lisele tse rarahaneng tseo mekhoa e tloaelehileng ea ho thibela motlakase e ke keng ea li khetholla, e fana ka tlhahlobo e tšepahalang ea lisele tse phatlohang le li-precursors tse sa pheleng.
Mocha oa ho lemoha
Mosebetsi o ka sehloohong oa litsi tsa dae ona o ho mocha oa WPC (White Cell Progenitor), o arolang li-leucocyte tsa khale ho lisele tse hōlileng tsebong.
Lenaneo la WPC:Fluorescent nucleic acid staining bakeng sa phapang ea lisele tsa pele, ho phatloha le li-lymphocyte tse sa tloaelehang.
WDF Channel:Boemo bo fapaneng ba litšila bakeng sa li-neutrophils tse holileng, lymphocyte, monocyte le eosinophils.
Channel ea WNR:Sistimi ea ho arabela e inehetseng ho boitsebahatso ba lisele tse khubelu tsa mali (NRBC) le lipalo tse felletseng tsa lisele tse tšoeu.
RET Channel:Leibole ea Nucleic acid bakeng sa tlhahlobo ea molumo oa reticulocyte le index ea kholo ea kholo.
Channel ea PLT:Specific fluorescence tagging for high-palo e nepahetseng ea optical platelet ka lisampole tse rarahaneng.
Letoto la Analyzer le lumellanang
- Automated 5-Part Hematology Analyzer X-Series
- Automated 5-Part Hematology Analyzer Y-Series
- Multi{{0}Channel Clinical Flow Hematology Systems
Litlhaloso tsa Sehlahisoa
|
Tekheniki Parameter |
Maemo a Tlhaloso |
|
Nomoro ea lethathamo |
STAC{{0}DYE{1}SYSWPC |
|
Sebopeho sa 'Mele |
Mokelikeli o hlakileng oa tharollo |
|
Boemo ba pH |
6.80 - 7.40 ka likhato tse 25 |
|
Absorbance Peak ($\\lambda_{max}$) |
635 nm - 645 nm |
|
Sepheo sa Channel |
WPC (White Cell Progenitor) |
|
Mokhoa oa ho Khetholla |
Semiconductor Laser Phallo Cytometry |
|
Tse ngata-ho-Lot CV |
Ka tlase kapa ho lekana le 2.5% |
|
Ho-Botsitso ba Boto |
Matsatsi a 60-ho bula |
|
Bophelo ba Shelf bo sa Buloang |
Likhoeli tse 18 |
|
Mocheso oa polokelo |
2℃- 30℃(Sireletsa leseli) |
Sesebelisoa le Mosebetsi oa Reagent
Letšoao le sa tloaelehang la Lymphocyte:E kenella ka har'a meaho ea cellular ho tšoaea intracellular RNA/DNA, e hlahisa likhokahano tse ikhethang tsa scattergram bakeng sa batho ba atypical lymphoid.
Identification ea Blast Cell:E theha meeli e hlakileng ea matla a fluorescence lipakeng tsa li-leukocyte tse holileng le-lisele tsa pele tsa ho phatloha.
Boima ba Granulocyte Quantification:E thusa ho fumana hantle ha myelocyte, metamyelocyte, le promyelocyte ka har'a tatellano ea tekanyo ea WPC.
Sephuthelo/ Bobolokelo
Sephutheloana se Tloaelehileng:12 $\\times$ 12 mL khanya-likhatriji tse sirelelitsoeng lebokose le leng.
Likhetho tsa ho Fana ka Bongata:Litlhophiso tse ngata- tsa li-cartridge batch li fumaneha bakeng sa-bolumo bo phahameng ba tšebetso ea laboratori ea boiketsetso.
Polokelo & Lipalangoang:Boloka sebakeng se omeletseng ho 2℃- 30℃. Lula u tiisitsoe ka thata 'me u sirelelitsoe ho pepesehela khanya e tobileng. Se hatsele. E tsitsitse tlas'a maemo a tloaelehileng a lipalangoang tsa mocheso.
Related Hematology Reagents
SYSWNR Dae ea Fluorescent:Reagent bakeng sa palo eohle ea lisele tse tšoeu tsa mali le lisele tse khubelu tsa mali tsa optical.
SYSWDF Dae ea Fluorescent:Sesebelisoa sa Fluorochrome bakeng sa tlhahlobo e tloaelehileng ea likarolo tse 5 tsa leukocyte.
SYSRET Dae ea Fluorescent:Dae e inehetseng ea nucleic acid bakeng sa liprothokholo tsa tekanyo ea reticulocyte.
SYSPLT Dae ea Fluorescent:Letheba le khethehileng la optical bakeng sa qeto ea optical platelet.
LBH
P: Mosebetsi oa tekheniki oa mocha oa ho lemoha oa WPC ke ofe?
A: Seteishene sa WPC se kopanya semiconductor laser flow cytometry le dae ea fluorescent nucleic acid ho khetholla lisele tsa pele tsa pele, ho phatloha, le li-lymphocyte tse sa tloaelehang ho tsoa ho mela e holileng ea lisele tse tšoeu tsa mali.
P: Reagent ee e fapana joang le matheba a tloaelehileng a smear ea mali a sa bonahaleng?
A: Matheba a microscopic a itšetlehile ka ho monya mebala bakeng sa tlhahlobo ea pono tlas'a khanya ea lebala le khanyang. Sehlahisoa sena ke fluorochrome ea mokelikeli e entsoeng ka mokhoa oa automated fluidic aspiration, cell membrane permeation, le ho lemoha{1}speed fluorescence ka har'a likanale tsa flow cytometry.
P: Litlhaloso tsa polokelo le meeli ea botsitso ea ts'ebetso ke efe?
K: Likhatriji tse sa buloang li boloka botsitso ba lik'hemik'hale ka botlalo ka likhoeli tse 18 ha li bolokiloe pakeng tsa 2℃le 30℃sebakeng se- se sireletsehileng. Ha e se e kentsoe sistimi, reagent e boloka ts'ebetso e felletseng ea matsatsi a 60.
P: Ke mekhoa efe ea ho laola boleng e tiisang hore-ho tla ba le{1}}ho lumellana ho hong?
K: Karolo e 'ngoe le e 'ngoe ea tlhahiso e etsa netefatso ea spectrophotometric peak absorbance, measurement pH, background fluorescence lerata le netefatso ea cell gating ho netefatsa hore{0}}ho{1}}lot coefficient of variation (CV) ka tlase ho 2.5%.
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